Utilizing a microfluidic approach for high-throughput synthesis of drug encapsulated Nanoliposomes

Abstract

Microfluidic platform was utilized for producing nanoliposomes using egg phosphatidylcholine (E-PC) and cholesterol (CH) by efficiently leveraging the mixing properties of microfluidic channels in a range of designs, including ‘Serpentine’, ‘Solid serpentine’, and ‘Koch fractal’. These microfluidic channels were made with computer-aided design (CAD) software and then transferred onto acrylic sheets using a CO2 laser. Along with carefully assessing the relative sizes and topologies of the channels, liquid flow parameters, and initial lipid concentrations, the characteristics of nanoliposomes were investigated using small-angle neutron scattering (SANS), dynamic light scattering (DLS), and high resolution transmission electron microscopy (HR-TEM). We were able to control the nanoliposomes dimensions within the preferred range (hydrodynamic diameter: 70–200 nm, PDI: less than 0.3). The operational framework of the proposed methodologies and the acquired results were readily adapted to the continuous, fast, and regulated creation of the nanoliposomes. Additionally, anticancer medications such as doxorubicin and genistein were successfully encapsulated in nanoliposomes, which demonstrated efficacious activity.